RNA

Part:BBa_K4830009:Design

Designed by: Beibitkyzy Aruzhan   Group: iGEM23_NTU-Singapore   (2023-10-10)


POAG snp1-3 ngRNA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design of ngRNA includes the U6 promoter, spacer, guide RNA scaffold, and polyA tail. The unique ngRNA was designed based on SNP data.

Source

SNPs were retrieved from the paper cited in References.

References

Gharahkhani, P. et al. (2021) ‘Genome-wide meta-analysis identifies 127 open-angle glaucoma loci with consistent effect across ancestries’, Nature Communications, 12(1). doi:10.1038/s41467-020-20851-4.